Back to Search
Start Over
Critical role for caspases 3 and 8 in neutrophil but not eosinophil apoptosis.
- Source :
-
International archives of allergy and immunology [Int Arch Allergy Immunol] 2001 Oct; Vol. 126 (2), pp. 147-56. - Publication Year :
- 2001
-
Abstract
- Background: Apoptosis is a necessary process to control cell numbers in multicellular organisms. In many chronic inflammatory diseases, reduced cell death of different types of granulocytes is one important mechanism for cell accumulation. Here, we studied the role of caspases in neutrophil and eosinophil apoptosis in the presence or absence of granulocyte-macrophage stimulating factor and anti-CD95 monoclonal antibodies, respectively.<br />Methods: Granulocytes were isolated from human blood using standard protocols. Immunoblot and functional studies with cell-permeable specific peptide inhibitors were performed to analyze caspase involvement. Fas receptor and Fas ligand expression was analyzed by RT-PCR, flow cytometry, and immunoblotting. Cell death was analyzed by ethidium bromide exclusion test.<br />Results: Caspases 3 and 8 are critically involved in the regulation of neutrophil apoptosis in vitro. In contrast, these two caspases did not appear to play a major role in the regulation of eosinophil apoptosis. However, the broad-range caspase inhibitor VAD prevented eosinophil death, indicating that caspases are also involved within the apoptotic machinery of eosinophils. Functional inhibitor studies suggested that caspase 9 is crucial for both caspase 3 and 8 activation, at least in neutrophils. In contrast, spontaneous apoptosis of neutrophils or eosinophils is unlikely to be the consequence of Fas ligand/Fas receptor molecular interactions.<br />Conclusion: The data of this study indicate differences in the usage of caspases between neutrophils and eosinophils.<br /> (Copyright 2001 S. Karger AG, Basel)
- Subjects :
- Antibodies, Monoclonal immunology
Caspase 3
Caspase 8
Caspase 9
Caspase Inhibitors
Cells, Cultured
Enzyme Activation
Eosinophils enzymology
Granulocyte-Macrophage Colony-Stimulating Factor pharmacology
Humans
Neutrophil Activation
Neutrophils enzymology
fas Receptor immunology
fas Receptor metabolism
Apoptosis physiology
Caspases metabolism
Eosinophils physiology
Neutrophils physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1018-2438
- Volume :
- 126
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International archives of allergy and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 11729353
- Full Text :
- https://doi.org/10.1159/000049506