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The role of antigen in the localization of naive, acutely activated, and memory CD8(+) T cells to the lung during influenza pneumonia.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2001 Dec 15; Vol. 167 (12), pp. 6983-90. - Publication Year :
- 2001
-
Abstract
- The role of Ag in the recruitment and localization of naive, acutely activated, and memory CD8(+) T cells to the lung during influenza infection was explored using TCR-transgenic (Tg) mice. Naive, Thy1.2(+)CD8(+) OT-I TCR-Tg cells were primed and recruited to the lung after transfer into congenic Thy1.1(+) recipients challenged with a genetically engineered influenza virus (influenza A/WSN/33 (WSN)-OVA(I)) containing the K(b) restricted OVA(257-264) epitope (siinfekl) in the viral neuraminidase stalk. However, if the transferred animals were infected with a similar influenza virus that expressed an irrelevant K(b) epitope (WSN-PEPII), no TCR-Tg T cells were detectable in the lung, although they were easily visible in the lymphoid organs. Conversely, there were substantial numbers of OT-I cells found in the lungs of WSN-PEPII-infected mice when the animals had been previously, or were concurrently, infected with a recombinant vaccinia virus expressing OVA. Similar results were obtained with nontransgenic populations of memory CD8(+) T cells reactive to a murine gamma-herpesvirus-68 Ag. Interestingly, the primary host response to the immunodominant influenza nucleoprotein epitope was not affected by the presence of memory or recently activated OT-I T cells. Thus, although Ag is required to activate the T cells, the subsequent localization of T cells to the lung during a virus infection is a property of recently activated and memory T cells and is not necessarily driven by Ag in the lung.
- Subjects :
- Adoptive Transfer
Animals
CD8-Positive T-Lymphocytes transplantation
Cells, Cultured
DNA, Recombinant administration & dosage
DNA, Viral genetics
Egg Proteins immunology
Female
Genes, T-Cell Receptor
Immunologic Memory
Influenza A virus genetics
Influenza A virus immunology
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Mice, Transgenic
Ovalbumin immunology
Peptide Fragments
Rhadinovirus immunology
Antigens, Viral immunology
CD8-Positive T-Lymphocytes immunology
Cell Movement
Lung immunology
Orthomyxoviridae Infections immunology
Pneumonia, Viral immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 167
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 11739518
- Full Text :
- https://doi.org/10.4049/jimmunol.167.12.6983