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Progastrin processing differs in 7B2 and PC2 knockout animals: a role for 7B2 independent of action on PC2.
- Source :
-
FEBS letters [FEBS Lett] 2002 Jan 02; Vol. 510 (1-2), pp. 89-93. - Publication Year :
- 2002
-
Abstract
- Cellular synthesis of neuroendocrine peptides requires prohormone convertases (PCs). In order to determine the role of PC2 for gastrin synthesis, we examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and alpha-amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K(53)-K(54) site was almost fully cleaved in controls and half cleaved in PC2 null mice, only 23% was cleaved in 7B2 null mice. The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin-17), and that 7B2 exhibits effects beyond PC2-mediated cleavages.
- Subjects :
- Animals
Chromatography, Gel methods
Female
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Molecular Chaperones genetics
Nerve Tissue Proteins genetics
Neuroendocrine Secretory Protein 7B2
Pituitary Hormones genetics
Proprotein Convertase 2
Protein Processing, Post-Translational
Subtilisins genetics
Gastrins metabolism
Molecular Chaperones physiology
Nerve Tissue Proteins physiology
Neurosecretory Systems metabolism
Pituitary Hormones physiology
Protein Precursors metabolism
Subtilisins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 510
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 11755537
- Full Text :
- https://doi.org/10.1016/s0014-5793(01)03234-3