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Comparison of antioxidant effects of naringin and probucol in cholesterol-fed rabbits.
- Source :
-
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2002 Mar; Vol. 317 (1-2), pp. 181-90. - Publication Year :
- 2002
-
Abstract
- Background: Due to the strong evidence on the involvement of active oxygen species in a variety of disorders, the role of antioxidants against oxidative stress has recently received increased attention.<br />Methods: Twenty male rabbits were served a high-cholesterol (HC, 5 g/kg diet) diet or high-cholesterol diet supplemented with naringin (0.5 g/kg diet) or probucol (0.5 g/kg diet) for 8 weeks to compare the antioxidative effects of the citrus bioflavonoid (naringin) and antioxidative cholesterol-lowering drug (probucol).<br />Results: The plasma thiobarbituric acid-reactive substances (TBARS) concentration was not significantly different between the groups, whereas the hepatic TBARS concentration was significantly lower in the probucol group than in both normal and HC control or naringin group. Probucol and naringin supplementation led to an increase in the hepatic superoxide dismutase (SOD) and catalase (CAT) activities, and a decrease in the hepatic mitochondrial hydrogen peroxide (H(2)O(2)) content compared to the HC-control group. However, there was no difference in the cytosolic H(2)O(2) content or cytosolic glutathion peroxidase (GSH-Px) activity in the liver between the groups. Both naringin and probucol supplements significantly increased the plasma vitamin E concentration compared to the HC-control group. As regards the antioxidant enzyme gene expressions, naringin significantly increased the expression of three antioxidant enzyme mRNAs compared to the HC-control group, whereas probucol significantly increased the only SOD mRNA expression.<br />Conclusions: The probucol supplement was very potent in the antioxidative defense system, whereas naringin exhibited a comparable antioxidant capacity based on increasing the gene expressions in the antioxidant enzymes, while also increasing the hepatic SOD and CAT activities, sparing plasma vitamin E, and decreasing the hepatic mitochondrial H(2)O(2) content.
- Subjects :
- Animals
Anticholesteremic Agents pharmacology
Cholesterol blood
Cholesterol pharmacology
Dietary Fats pharmacology
Eating drug effects
Enzymes drug effects
Enzymes genetics
Enzymes metabolism
Gene Expression Regulation, Enzymologic drug effects
Hydrogen Peroxide metabolism
Lipid Peroxidation drug effects
Liver anatomy & histology
Liver metabolism
Male
Organ Size drug effects
Rabbits
Vitamin A blood
Vitamin E blood
Weight Gain drug effects
Antioxidants pharmacology
Flavanones
Flavonoids pharmacology
Liver drug effects
Probucol pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0009-8981
- Volume :
- 317
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Clinica chimica acta; international journal of clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 11814474
- Full Text :
- https://doi.org/10.1016/s0009-8981(01)00778-1