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Identification of major outer surface proteins of Streptococcus agalactiae.
- Source :
-
Infection and immunity [Infect Immun] 2002 Mar; Vol. 70 (3), pp. 1254-9. - Publication Year :
- 2002
-
Abstract
- To identify the major outer surface proteins of Streptococcus agalactiae (group B streptococcus), a proteomic analysis was undertaken. An extract of the outer surface proteins was separated by two-dimensional electrophoresis. The visualized spots were identified through a combination of peptide sequencing and reverse genetic methodologies. Of the 30 major spots identified as S. agalactiae specific, 27 have been identified. Six of these proteins, previously unidentified in S. agalactiae, were sequenced and cloned. These were ornithine carbamoyltransferase, phosphoglycerate kinase, nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, purine nucleoside phosphorylase, enolase, and glucose-6-phosphate isomerase. Using a gram-positive expression system, we have overexpressed two of these proteins in an in vitro system. These recombinant, purified proteins were used to raise antisera. The identification of these proteins as residing on the outer surface was confirmed by the ability of the antisera to react against whole, live bacteria. Further, in a neonatal-animal model system, we demonstrate that some of these sera are protective against lethal doses of bacteria. These studies demonstrate the successful application of proteomics as a technique for identifying vaccine candidates.
- Subjects :
- Amino Acid Sequence
Animals
Animals, Newborn
Antibodies, Bacterial immunology
Bacterial Outer Membrane Proteins immunology
Electrophoresis, Gel, Two-Dimensional
Immunization, Passive
Mice
Molecular Sequence Data
Ornithine Carbamoyltransferase immunology
Phosphoglycerate Kinase immunology
Proteome
Sequence Analysis, Protein
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Streptococcal Infections etiology
Streptococcal Infections prevention & control
Streptococcus agalactiae immunology
Bacterial Outer Membrane Proteins isolation & purification
Streptococcus agalactiae chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 70
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 11854208
- Full Text :
- https://doi.org/10.1128/IAI.70.3.1254-1259.2002