Back to Search
Start Over
Intravirion display of a peptide corresponding to the dimer structure of protease attenuates HIV-1 replication.
- Source :
-
DNA and cell biology [DNA Cell Biol] 2001 Dec; Vol. 20 (12), pp. 797-805. - Publication Year :
- 2001
-
Abstract
- Current treatment of HIV-1-infected individuals involves the administration of several drugs, all of which target either the reverse transcriptase or the protease activity of the virus. Unfortunately, the benefits of such treatments are compromised by the emergence of viruses exhibiting resistance to the drugs. This situation warrants new approaches for interfering with virus replication. Considering the activation of protease in the virus particles, a novel strategy to inhibit HIV-1 replication was tested targeting the dimerization domain of the protease. To test this idea, we have selected four residues from the C terminus of HIV-1 protease that map to the dimer interface region of the enzyme. We have exploited Vpr to display the peptides in the virus particles. The chimeric Vpr exhibited expression and virion incorporation similar to wildtype Vpr. The virus derived from the HIV-1 proviral DNA containing chimeric Vpr sequences registered a reduced level of replication in CEM and CEM X 174 cells in comparison with viruses containing wildtype Vpr. Similar results were observed in a single-round replication assay. These results suggest that the intravirion display of peptides targeting viral proteins is a powerful approach for developing antiviral agents and for dissecting the dynamic interactions between structural proteins during virus assembly and disassembly.
- Subjects :
- Base Sequence
Cell Line
Gene Products, vpr chemistry
Humans
Molecular Sequence Data
Protein Biosynthesis
Radioimmunoprecipitation Assay
Recombinant Fusion Proteins chemistry
Recombinant Proteins pharmacology
vpr Gene Products, Human Immunodeficiency Virus
Anti-HIV Agents pharmacology
Gene Products, vpr pharmacology
HIV Protease metabolism
HIV-1 physiology
Recombinant Fusion Proteins pharmacology
Virus Replication drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1044-5498
- Volume :
- 20
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- DNA and cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 11879573
- Full Text :
- https://doi.org/10.1089/104454901753438615