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Novel G-protein complex whose requirement is linked to the translational status of the cell.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 2002 Apr; Vol. 22 (8), pp. 2564-74. - Publication Year :
- 2002
-
Abstract
- G proteins, which bind and hydrolyze GTP, are involved in regulating a variety of critical cellular processes, including the process of protein synthesis. Many members of the subfamily of elongation factor class G proteins interact with the ribosome and function to regulate discrete steps during the process of protein synthesis. Despite sequence similarity to factors involved in translation, a role for the yeast Hbs1 protein has not been defined. In this work we have identified a genetic relationship between genes encoding components of the translational apparatus and HBS1. HBS1, while not essential for viability, is important for efficient growth and protein synthesis under conditions of limiting translation initiation. The identification of an Hbs1p-interacting factor, Dom34p, which shares a similar genetic relationship with components of the translational apparatus, suggests that Hbs1p and Dom34p may function as part of a complex that facilitates gene expression. Dom34p contains an RNA binding motif present in several ribosomal proteins and factors that regulate translation of specific mRNAs. Thus, Hbs1p and Dom34p may function together to help directly or indirectly facilitate the expression either of specific mRNAs or under certain cellular conditions.
- Subjects :
- Amino Acid Sequence
Amino Acid Substitution
Base Sequence
Cell Cycle Proteins chemistry
Cell Cycle Proteins genetics
Cell Cycle Proteins metabolism
Endoribonucleases
Fungal Proteins chemistry
Fungal Proteins genetics
GTP-Binding Proteins chemistry
GTP-Binding Proteins genetics
Gene Deletion
Gene Expression
Genes, Fungal
Genetic Complementation Test
HSP70 Heat-Shock Proteins chemistry
HSP70 Heat-Shock Proteins genetics
Macromolecular Substances
RNA Processing, Post-Transcriptional
RNA, Fungal genetics
RNA, Fungal metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Ribosomes metabolism
Saccharomyces cerevisiae growth & development
Saccharomyces cerevisiae Proteins chemistry
Saccharomyces cerevisiae Proteins genetics
Fungal Proteins metabolism
GTP-Binding Proteins metabolism
HSP70 Heat-Shock Proteins metabolism
Peptide Elongation Factors
Protein Biosynthesis
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0270-7306
- Volume :
- 22
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 11909951
- Full Text :
- https://doi.org/10.1128/MCB.22.8.2564-2574.2002