Back to Search
Start Over
Redundant and unique roles of two enhancer elements in the TCRgamma locus in gene regulation and gammadelta T cell development.
- Source :
-
Immunity [Immunity] 2002 Mar; Vol. 16 (3), pp. 453-63. - Publication Year :
- 2002
-
Abstract
- Many mammalian genes, including those encoding antigen receptors, contain more than one enhancer element. Deleting one element often does not prevent expression, but functional redundancy has never been directly demonstrated by gene targeting of multiple elements. We demonstrate that simultaneous deletion of two enhancer/LCR-like elements in the TCR Cgamma1 cluster, HsA and 3'E(Cgamma1), severely diminishes TCRgamma transcription, selectively impairs development of gammadelta thymocyte subsets, but only modestly reduces TCRgamma gene rearrangement, while deletion of each element separately has little effect. In contrast to these results in thymocytes, deletion of HsA alone reduces transcription of one Vgamma gene specifically in peripheral gammadelta T cells. Thus, the two elements exhibit functional redundancy in thymocytes but also have unique functions in other settings.
- Subjects :
- Animals
Gene Expression Regulation immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Antigen, T-Cell, gamma-delta immunology
Recombination, Genetic
Enhancer Elements, Genetic genetics
Genes, T-Cell Receptor gamma genetics
Receptors, Antigen, T-Cell, gamma-delta genetics
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1074-7613
- Volume :
- 16
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Immunity
- Publication Type :
- Academic Journal
- Accession number :
- 11911829
- Full Text :
- https://doi.org/10.1016/s1074-7613(02)00285-6