Back to Search
Start Over
Mechanism of murine Vgamma1+ gamma delta T cell-mediated innate immune response against Listeria monocytogenes infection.
- Source :
-
European journal of immunology [Eur J Immunol] 2002 Apr; Vol. 32 (4), pp. 928-35. - Publication Year :
- 2002
-
Abstract
- Murine gamma delta T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the gamma delta T cell-mediated response against L. monocytogenes infection. gamma delta T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-gamma in vitro in response to L. monocytogenes-infected spleen cells. The IFN-gamma production was abrogated by depletion of Vgamma1+ gamma delta T cells. IFN-gamma production of the Vgamma1+ gamma delta T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR gamma delta monoclonal antibodies (mAb) failed to block the response, suggesting that the response requires no TCR-mediated antigen recognition. Interestingly, Vgamma1+ gamma delta T cells of naive mice also produced IFN-gamma in response to L. monocytogenes-infected spleen cells in an IL-12-dependent manner. Furthermore, the IL-12 receptor (IL-12R) gene was expressed on the Vgamma1+ gamma delta T cells of naive mice as well as those of L. monocytogenes-infected mice although naive alpha beta T cells lack IL-12R expression. All the results suggest that the Vgamma1+ gamma delta T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-gamma in response to infection-induced IL-12 without antigen-driven clonal expansion and maturation.
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Antibodies, Monoclonal pharmacology
Cells, Cultured drug effects
Cells, Cultured immunology
Immunity, Cellular
Immunity, Innate
Immunoglobulin Fab Fragments immunology
Immunoglobulin Fab Fragments pharmacology
Interferon-gamma metabolism
Interleukin-12 pharmacology
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Receptors, Antigen, T-Cell, gamma-delta genetics
Receptors, Interleukin biosynthesis
Receptors, Interleukin-12
Spleen cytology
T-Lymphocyte Subsets drug effects
T-Lymphocyte Subsets metabolism
Listeria monocytogenes immunology
Listeriosis immunology
Receptors, Antigen, T-Cell, gamma-delta immunology
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 32
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 11920558
- Full Text :
- https://doi.org/10.1002/1521-4141(200204)32:4<928::AID-IMMU928>3.0.CO;2-I