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Cutting edge: TCR engagement and triggering in the absence of large-scale molecular segregation at the T cell-APC contact site.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2002 May 01; Vol. 168 (9), pp. 4287-91. - Publication Year :
- 2002
-
Abstract
- We investigated the functional role of large-scale molecular segregation at the T cell-APC contact site during T lymphocyte Ag recognition. Inhibition of CD2-CD58 interaction markedly affected segregation of CD2 and CD2AP from CD45. Under these conditions, Ag-induced calcium mobilization, PKC theta; clustering at the immunological synapse, and IFN-gamma production also were inhibited. However, early TCR signaling and T cell polarization toward APCs were unaffected. Our results indicate that the "raison d'être" of a large-scale segregation of surface molecules and intracellular enzymes and adapters, in Ag-stimulated T cells, is to reinforce the assembly of the signal transduction cascade rather than favor TCR engagement and triggering.
- Subjects :
- Adaptor Proteins, Signal Transducing
CD2 Antigens physiology
CD58 Antigens metabolism
Cell Line, Transformed
Cell Polarity
Clone Cells
Cytoskeletal Proteins
Interferon-gamma biosynthesis
Macromolecular Substances
Proteins metabolism
Signal Transduction
T-Lymphocytes ultrastructure
Antigen-Presenting Cells immunology
Receptors, Antigen, T-Cell immunology
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 168
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 11970969
- Full Text :
- https://doi.org/10.4049/jimmunol.168.9.4287