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[Protective effect of low concentration endothelin-1 on the reactive oxygen-induced inhibition of pulmonary surfactant lipid synthesis].
- Source :
-
Sheng li xue bao : [Acta physiologica Sinica] [Sheng Li Xue Bao] 2002 Apr 25; Vol. 54 (2), pp. 89-93. - Publication Year :
- 2002
-
Abstract
- The effects of endothelin-1 (ET-1) at low concentration (1-100 pmol/L) on the reactive oxygen-induced inhibition of both pulmonary surfactant (PS) lipid synthesis and the activity of CTP: phosphorylcholine cytidylyltransferase (CCT), a rate-limiting enzyme in biosynthesis of phosphoatidylcholine (PC), were studied in cultured lung explants without serum. The xanthine-xanthine oxidase superoxide anion generating system decreased (3)H-choline incorporation into PC in a dose-dependent manner in cultured lung explants. ET-1 reduced both the reactive oxygen-induced decrease in (3)H-choline incorporation and the increase in malondialdehyde (MDA) content of lung tissues, but did not change the levels of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and the total antioxidant capability in the lung explants. ET-1 enhanced microsomal CCT activity of the lung tissues, while it decreased cytosolic CCT activity of lung tissues. ET-1 also prevented the inhibitive effect of reactive oxygen on microsomal CCT activity in the lung explants. These results suggest that ET-1 at low concentration can protect the microsomal CCT activity and reduce the inhibition of PS lipid synthesis induced by oxidant lung injury. The protective mechanism of ET-1 is not relative to the pulmonary endogenous antioxidant defense system.
- Subjects :
- Animals
Choline-Phosphate Cytidylyltransferase metabolism
Endothelin-1 pharmacology
Female
In Vitro Techniques
Lung drug effects
Lung enzymology
Male
Rats
Rats, Wistar
Endothelin-1 administration & dosage
Lung metabolism
Phospholipids biosynthesis
Pulmonary Surfactants chemistry
Reactive Oxygen Species toxicity
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0371-0874
- Volume :
- 54
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Sheng li xue bao : [Acta physiologica Sinica]
- Publication Type :
- Academic Journal
- Accession number :
- 11973583