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Comparative analysis of two coxsackievirus B3 strains: putative influence of virus-receptor interactions on pathogenesis.
- Source :
-
Journal of medical virology [J Med Virol] 2002 Jun; Vol. 67 (2), pp. 224-33. - Publication Year :
- 2002
-
Abstract
- Strain-specific differences in the interaction of coxsackievirus B3 (CVB3) with the coxsackievirus-adenovirus receptor (CAR) and the decay-accelerating factor (DAF) co-receptor proteins were investigated using a non-haemagglutinating (CVB3) and a haemagglutinating (CVB3-HA) strain of CVB3. A panel of receptor-transfected hamster CHO cells, expressing either CAR (CHOCAR cells), DAF (CHODAF cells), or both receptor proteins (CHODC cells) were used to study the interplay of CAR and DAF receptor molecules with regard to binding and infection with CVB3 and CVB3-HA. Despite clear differences in their binding phenotypes, both virus strains were found to primarily depend on the CAR receptor protein for initialization of productive infections. Cytopathic effects induced by CVB3-HA were influenced by co-expression of DAF receptor proteins. The cardiotropic potential of both virus strains was investigated in A.BY/SnJ mice. Despite comparable virus replication of both CVB3 strains in individual myocytes, the number of infected heart muscle cells was significantly lower in CVB3-HA infected mice. Infections of pancreata correlated with myocardial infections. Together these data suggest that even small differences in virus-receptor interactions, influencing virus binding and virus spread, may have an impact on the pathogenesis of CVB-induced diseases.<br /> (Copyright 2002 Wiley-Liss, Inc.)
- Subjects :
- Animals
CD55 Antigens genetics
CHO Cells
Coxsackie and Adenovirus Receptor-Like Membrane Protein
Cricetinae
Enterovirus B, Human metabolism
HeLa Cells
Humans
Mice
Myocarditis physiopathology
Myocarditis virology
Pancreatitis physiopathology
Pancreatitis virology
Receptors, Virus genetics
Transfection
CD55 Antigens metabolism
Enterovirus B, Human classification
Enterovirus B, Human pathogenicity
Receptors, Virus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0146-6615
- Volume :
- 67
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of medical virology
- Publication Type :
- Academic Journal
- Accession number :
- 11992583
- Full Text :
- https://doi.org/10.1002/jmv.2211