Back to Search
Start Over
Inhibitory effects of cytomegalovirus proteins US2 and US11 point to contributions from direct priming and cross-priming in induction of vaccinia virus-specific CD8(+) T cells.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2002 Jun 01; Vol. 168 (11), pp. 5403-8. - Publication Year :
- 2002
-
Abstract
- The extent to which naive CD8(+) CTLs (T(CD8)(+)) are primed by APCs presenting endogenous Ags (direct priming) or Ags acquired from other infected cells (cross-priming) is a critical topic in basic and applied immunology. To examine the contribution of direct priming in the induction of VV-specific T(CD8)(+), we generated recombinant vaccinia viruses that express human CMV proteins (US2 and US11) that induce the destruction of newly synthesized MHC class I molecules. Expression of US2 or US11 was associated with a 24-63% decrease in numbers of primary or secondary VV-specific T(CD8)(+) responding to i.p. infection. Using HPLC-isolated peptides from VV-infected cells, we show that US2 and US11 selectively inhibit T(CD8)(+) responses to a subset of immunogenic VV determinants. Moreover, VV-US2 and lysates from VV-infected histoincompatible cells elicit T(CD8)(+) specific for a similar subset of VV determinants. These findings indicate that US2 and US11 can function in vivo to interfere with the activation of virus-specific T(CD8)(+). Furthermore, they suggest that 1) both cross-priming and direct priming contribute significantly to the generation of VV-specific T(CD8)(+), 2) the sets of immunogenic vaccinia virus determinants generated by cross-priming and direct priming are not completely overlapping, and 3) cross-priming overrides the effects of cis-acting viral interference with the class I Ag presentation pathway.
- Subjects :
- Animals
CD4-Positive T-Lymphocytes physiology
Female
H-2 Antigens analysis
H-2 Antigens physiology
Histocompatibility Antigen H-2D
Interferon-gamma biosynthesis
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Tumor Necrosis Factor-alpha biosynthesis
CD8-Positive T-Lymphocytes immunology
RNA-Binding Proteins physiology
Vaccinia virus immunology
Viral Envelope Proteins physiology
Viral Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 168
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 12023332
- Full Text :
- https://doi.org/10.4049/jimmunol.168.11.5403