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A novel gene IC53 stimulates ECV304 cell proliferation and is upregulated in failing heart.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2002 May 31; Vol. 294 (1), pp. 161-6. - Publication Year :
- 2002
-
Abstract
- C53, cloned from rat brain cDNA library, can bind to p35, the precursor of activator of Cdk5. A novel gene with 84% homolog to C53, named IC53, was cloned from our 5300 EST database of human aorta cDNA library (GenBank Accession No. AF110322). Computational analysis showed that IC53 cDNA is 2538 bp long, encoding 419 amino acids, mapped to chromosome 17q21.31 with 12 exons, ubiquitously expressed in 12 tested normal tissues and 8 tumor cell lines from MTN membranes and vascular endothelial cells by Northern blot and in situ hybridization, and upregulated in the rat models of subacute heart failure and chronic ischemic heart failure by left coronary ligation. Stable transfection of IC53 stimulates ECV304 cell proliferation by 2.1-fold compared to cells with empty vector (P<0.05). The results support that IC53 is a novel gene, mainly expressed in vascular endothelial cells and mediates cell proliferation.
- Subjects :
- Amino Acid Sequence
Animals
Aorta chemistry
Base Sequence
Carrier Proteins genetics
Carrier Proteins physiology
Cell Cycle Proteins
Cloning, Molecular
Endothelium, Vascular cytology
Endothelium, Vascular growth & development
Humans
In Situ Hybridization
Male
Molecular Sequence Data
Muscle Proteins physiology
Nerve Tissue Proteins genetics
Nerve Tissue Proteins physiology
Open Reading Frames
Rats
Rats, Sprague-Dawley
Tumor Cells, Cultured
Tumor Suppressor Proteins
Up-Regulation
Cell Division genetics
Chromosomes, Human, Pair 17
Intracellular Signaling Peptides and Proteins
Muscle Proteins genetics
Myocardial Ischemia pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 294
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 12054757
- Full Text :
- https://doi.org/10.1016/S0006-291X(02)00446-1