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Expression and localization of the cysteinyl leukotriene 1 receptor in human nasal mucosa.
- Source :
-
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology [Clin Exp Allergy] 2002 Jul; Vol. 32 (7), pp. 1007-12. - Publication Year :
- 2002
-
Abstract
- Background: The cysteinyl leukotrienes (CysLT) are lipid mediators that have been implicated in the pathogenesis of allergic diseases. Pharmacological studies using CysLTs indicate that two classes of receptors, named CysLT1 and CysLT2 receptor, exist. The former is sensitive to the CysLT1 antagonist currently used to treat asthma and allergic rhinitis. Recently, the cDNA for human CysLT1 and CysLT2 receptor have been cloned, making it now possible to study the gene expression of CysLTs receptors.<br />Objective: We have used reverse transcription and polymerase chain reaction (RT-PCR) to study the gene expression of CysLT1 and CysLT2 receptor and in situ hybridization to determine the distribution of CysLT1 receptor mRNA in human nasal mucosa. In addition, the distribution of the CysLT1 receptor protein was studied by immunohistochemistry.<br />Methods: Human turbinates were obtained after turbinectomy from six patients with nasal obstruction refractory to medical therapy. Total RNA was isolated from human nasal mucosa and both CysLT1 and CysLT2 receptor mRNA was detected in these tissues by using RT-PCR. For in situ hybridization study of human nasal mucosa, we used biotin-labelled oligonucleotides probes encoding human CysLT1 receptor cDNA. To identify the cells expressing the CysLT1 receptor protein, double immunostaining was performed by using anti-CysLT1 receptor antibody and monoclonal antileucocyte antibodies.<br />Results: RT-PCR analysis of total nasal RNA demonstrated the expression of both CysLT1 receptor and CysLT2 receptor mRNA. In situ hybridization indicated high levels of CysLT1 receptor hybridization in blood vessels and the interstitial cells, but a sparse signal in airway epithelium and submucosal glands. The immunohistochemical studies revealed that anti-CysLT1 receptor antibody labelled eosinophils, mast cells, macrophages, neutrophils and vascular endothelial cells in the nasal mucosa.<br />Conclusion: The results may have an important clinical implication and also promote further investigation of the regulation of CysLT1 receptor in health and disease.
- Subjects :
- Adolescent
Adult
Female
Humans
Immunohistochemistry
In Situ Hybridization
Leukotriene D4 pharmacology
Male
Middle Aged
Nasal Mucosa chemistry
Receptors, Leukotriene analysis
Reverse Transcriptase Polymerase Chain Reaction
Membrane Proteins
Nasal Mucosa metabolism
Receptors, Leukotriene genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0954-7894
- Volume :
- 32
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12100046
- Full Text :
- https://doi.org/10.1046/j.1365-2222.2002.01425.x