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Cloning, Sequencing and Expression of Human Copper, Zinc-superoxide Dismutase cDNA.
- Source :
-
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica [Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)] 1999; Vol. 31 (1), pp. 16-18. - Publication Year :
- 1999
-
Abstract
- The cDNA encoding the human copper, zinc-superoxide dismutase(Cu, Zn-SOD) was amplified from the human liver by RT-PCR and sequenced. The cloned human Cu, Zn-SOD cDNA was ligated into expression vector pET-22b(+) under T7 promotor. After 3 h induction with 1 mmol/L IPTG, human Cu, Zn-SOD was highly expressed in E.coli BL21(DE3). The expression product was up to 30% of the total protein of the bacteria in soluble form, which had specific SOD activity.
Details
- Language :
- English
- ISSN :
- 0582-9879
- Volume :
- 31
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 12142908