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Adhesion to bile drain materials and physicochemical surface properties of Enterococcus faecalis strains grown in the presence of bile.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2002 Aug; Vol. 68 (8), pp. 3855-8. - Publication Year :
- 2002
-
Abstract
- The aim of this study is to determine whether growth in the presence of bile influences the surface properties and adhesion to hydrophobic bile drain materials of Enterococcus faecalis strains expressing aggregation substance (Agg) or enterococcal surface protein (Esp), two surface proteins that are associated with infections. After growth in the presence of bile, the strains were generally more hydrophobic by water contact angles and the zeta potentials were more negative than when the strains were grown in the absence of bile. Nitrogen was found in lower surface concentrations upon growth in the presence of bile, whereas higher surface concentrations of oxygen were measured by X-ray photoelectron spectroscopy. Moreover, an up to twofold-higher number of bacteria adhered after growth in bile for E. faecalis not expressing Agg or Esp and E. faecalis with Esp on its surface. E. faecalis expressing Agg did not adhere in higher numbers after growth in bile, possibly because they mainly adhere through positive cooperativity and less through direct interactions with a substratum surface. Since adhesion of bacteria is the first step in biomaterial-centered infection, it can be concluded that growth in bile increases the virulence of E. faecalis.
- Subjects :
- Adhesins, Bacterial metabolism
Animals
Bacterial Proteins metabolism
Culture Media
Enterococcus faecalis chemistry
Enterococcus faecalis physiology
Humans
Hydrophobic and Hydrophilic Interactions
Membrane Proteins metabolism
Static Electricity
Bacterial Adhesion
Bile metabolism
Enterococcus faecalis growth & development
Surface Properties
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 68
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 12147481
- Full Text :
- https://doi.org/10.1128/AEM.68.8.3855-3858.2002