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External quality assessment program for Chlamydia trachomatis diagnostic testing by nucleic acid amplification assays.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2002 Aug; Vol. 40 (8), pp. 2893-6. - Publication Year :
- 2002
-
Abstract
- We report the results from 57 Australian diagnostic laboratories testing two external quality assessment panels using either the Roche Amplicor Chlamydia trachomatis test (R-PCR) or the Abbott LCx Chlamydia trachomatis assay (A-ligase chain reaction [LCR]). Panel samples were either normal urine spiked with Chlamydia trachomatis antigen or clinical urine specimens. There was no significant difference between laboratories or between assays in detection of C. trachomatis-positive clinical samples. Only at the lower limit of detection of the assays did the R-PCR demonstrate increased sensitivity over the A-LCR in the detection of C. trachomatis antigen. However, it was found that single-sample testing could lead to decreased test sensitivity. Detection of the presence of inhibitors of nucleic acid amplification differed between laboratories.
- Subjects :
- Australia
Chlamydia Infections microbiology
Chlamydia trachomatis genetics
Humans
Laboratories
Ligase Chain Reaction
Polymerase Chain Reaction
Quality Control
Sensitivity and Specificity
Urine microbiology
Chlamydia Infections diagnosis
Chlamydia trachomatis isolation & purification
Nucleic Acid Amplification Techniques methods
Reagent Kits, Diagnostic
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 40
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 12149347
- Full Text :
- https://doi.org/10.1128/JCM.40.8.2893-2896.2002