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Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12.
- Source :
-
Molecular microbiology [Mol Microbiol] 2002 Oct; Vol. 46 (1), pp. 281-91. - Publication Year :
- 2002
-
Abstract
- We have systematically examined the mRNA profiles of 36 two-component deletion mutants, which include all two-component regulatory systems of Escherichia coli, under a single growth condition. DNA microarray results revealed that the mutants belong to one of three groups based on their gene expression profiles in Luria-Bertani broth under aerobic conditions: (i) those with no or little change; (ii) those with significant changes; and (iii) those with drastic changes. Under these conditions, the anaeroresponsive ArcB/ArcA system, the osmoresponsive EnvZ/OmpR system and the response regulator UvrY showed the most drastic changes. Cellular functions such as flagellar synthesis and expression of the RpoS regulon were affected by multiple two-component systems. A high correlation coefficient of expression profile was found between several two-component mutants. Together, these results support the view that a network of functional interactions, such as cross-regulation, exists between different two-component systems. The compiled data are avail-able at our website (http://ecoli.aist-nara.ac.jp/xp_analysis/ 2_components).
- Subjects :
- Bacterial Proteins genetics
Escherichia coli growth & development
Escherichia coli physiology
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Proteome
Transcription, Genetic
Bacterial Proteins metabolism
Escherichia coli genetics
Gene Expression Regulation, Bacterial
Mutation
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 0950-382X
- Volume :
- 46
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 12366850
- Full Text :
- https://doi.org/10.1046/j.1365-2958.2002.03170.x