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E-selectin-inducing activity in plasma from type 2 diabetic patients with maculopathy.
- Source :
-
American journal of physiology. Endocrinology and metabolism [Am J Physiol Endocrinol Metab] 2003 Jan; Vol. 284 (1), pp. E1-6. Date of Electronic Publication: 2002 Aug 13. - Publication Year :
- 2003
-
Abstract
- Diabetic maculopathy (DMa) is a leading cause of visual loss in the western world. We examined whether plasma from type 2 diabetic patients with DMa contains factor(s) capable of inducing expression of the adhesion molecules E-selectin and VCAM-1 or cellular proliferation in cultured endothelial cells. Four gender-, age-, and duration (diabetes groups)-matched groups of 20 subjects each participated: 1) subjects with normal glucose tolerance (NGT), 2) subjects with impaired glucose tolerance (IGT), 3) type 2 diabetic patients without retinopathy, and 4) type 2 diabetic patients with DMa. Fasting plasma was added to in vitro-grown human umbilical vein endothelial cells for 6 h, after which E-selectin and VCAM-1 expression was measured. Proliferation was evaluated by thymidine incorporation. The individuals were characterized by measurement of 24-h ambulatory blood pressure, urinary albumin excretion rate, Hb A(1c), and blood lipids. Plasma from type 2 diabetic patients with DMa induced a significantly higher expression of E-selectin in endothelial cells than did plasma from subjects with NGT (259 +/- 23 x 10(3) vs. 198 +/- 19 x 10(3); arbitrary absorbance units; P < 0.05). There were no significant differences in plasma stimulatory effects on VCAM-1 expression or on thymidine incorporation between groups. These findings suggest that plasma from type 2 diabetic patients with DMa contains factor(s) capable of inducing the expression of E-selectin in endothelial cells. Enhanced expression of E-selectin may contribute to the development of DMa in type 2 diabetes.
- Subjects :
- Albuminuria
Blood
Blood Pressure
Cell Division drug effects
Cells, Cultured
DNA biosynthesis
E-Selectin analysis
Endothelium, Vascular metabolism
Female
Glucose Intolerance blood
Glycated Hemoglobin analysis
Humans
Lipids blood
Male
Middle Aged
Umbilical Veins
Vascular Cell Adhesion Molecule-1 analysis
Vascular Cell Adhesion Molecule-1 genetics
Diabetes Mellitus, Type 2 blood
Diabetic Retinopathy blood
E-Selectin genetics
Gene Expression drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0193-1849
- Volume :
- 284
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Endocrinology and metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 12388172
- Full Text :
- https://doi.org/10.1152/ajpendo.00198.2002