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Characterization of mac25/angiomodulin expression by high endothelial venule cells in lymphoid tissues and its identification as an inducible marker for activated endothelial cells.
- Source :
-
International immunology [Int Immunol] 2002 Nov; Vol. 14 (11), pp. 1273-82. - Publication Year :
- 2002
-
Abstract
- Previous results have indicated that mac25/angiomodulin (AGM) is expressed in lymph node (LN) high endothelial venules (HEV), the specialized venules that support efficient lymphocyte transendothelial migration. How mac25/AGM's endothelial expression pattern is regulated in situ remains unknown. Here, we demonstrate that in mouse LN blood vessels, including HEV, mac25/AGM is localized, unlike previous reports, not to the luminal or lateral regions bordering the endothelial cells (EC), but exclusively to the basal lamina that is in direct association with EC. In the spleen, mac25/AGM was expressed in the vascular basal lamina, in direct association with smooth muscle cells and pericytes, but not with EC. In addition, we report herein that mac25/AGM is an inducible marker for activated EC. In inflamed tissues, mac25/AGM expression was strongly induced in the abluminal region of blood vessels. In vitro, mac25/AGM was readily induced in EC upon activation with pro-inflammatory cytokines such as tumor necrosis factor-alpha, indicating that mac25/AGM is an activated EC marker. mac25/AGM binds vascular endothelial growth factor and, together with its strict abluminal localization, it is suggested that mac25/AGM has a specific function(s) in the subendothelium of activated blood vessels such as capturing humoral factors produced in the vicinity of HEV.
- Subjects :
- Animals
Biomarkers
Cell Line
Endothelial Growth Factors metabolism
Gene Expression Regulation
In Vitro Techniques
Intercellular Signaling Peptides and Proteins metabolism
Lymphoid Tissue metabolism
Lymphokines metabolism
Mice
Mice, Inbred BALB C
Neoplasm Proteins biosynthesis
Neoplasm Proteins metabolism
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Endothelium metabolism
Neoplasm Proteins genetics
Venules metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0953-8178
- Volume :
- 14
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12407018
- Full Text :
- https://doi.org/10.1093/intimm/dxf102