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Subunit gamma-green fluorescent protein fusions are functionally incorporated into mitochondrial F1F0-ATP synthase, arguing against a rigid cap structure at the top of F1.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2003 Jan 03; Vol. 278 (1), pp. 251-6. Date of Electronic Publication: 2002 Oct 31. - Publication Year :
- 2003
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Abstract
- We have investigated the question of the presence of a cap structure located at the top of the F(1) alpha(3)beta(3) hexamer of the yeast mitochondrial F(1)F(0)-ATP synthase complex. Specifically, we sought to determine whether the putative cap has a rigid structure and occludes the central shaft space formed by the alpha(3)beta(3) hexamer or alternatively whether the cap is more flexible permitting access to the central shaft space under certain conditions. Thus, we sought to establish whether subunit gamma, an essential component of the F(1) central stalk housed within the central shaft space and whose N and C termini would both lie beneath a putative cap, could be fused at its C terminus to green fluorescent protein (GFP) without loss of enzyme function. The GFP moiety serves to report on the integrity and location of fusion proteins containing different length polypeptide linkers between GFP and subunit gamma, as well as being a potential occluding structure in itself. Functional incorporation of subunit gamma-GFP fusions into ATP synthase of yeast cells lacking native subunit gamma was demonstrated by the ability of intact complexes to hydrolyze ATP and retain sensitivity to oligomycin. Our conclusion is that the putative cap structure cannot be an inflexible structure, but must be of a more flexible nature consistent with the accommodation of subunit gamma-GFP fusions within functional ATP synthase complexes.
- Subjects :
- Adenosine Triphosphate metabolism
Amino Acid Sequence
Animals
Base Sequence
Cattle
Cell Fractionation
Fungal Proteins chemistry
Fungal Proteins genetics
Fungal Proteins metabolism
Green Fluorescent Proteins
Luminescent Proteins genetics
Luminescent Proteins metabolism
Models, Molecular
Molecular Sequence Data
Protein Structure, Quaternary
Protein Subunits genetics
Protein Subunits metabolism
Proton-Translocating ATPases genetics
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Yeasts cytology
Yeasts enzymology
Yeasts physiology
Mitochondria metabolism
Protein Structure, Secondary
Proton-Translocating ATPases chemistry
Proton-Translocating ATPases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 278
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12414811
- Full Text :
- https://doi.org/10.1074/jbc.M204556200