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Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration.
- Source :
-
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica [Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)] 2002 Nov; Vol. 34 (6), pp. 703-6. - Publication Year :
- 2002
-
Abstract
- Strictosidine synthase (STR) is the key enzyme involved in early steps of biosynthesis of monoterpenoid indole alkaloids. STR catalyzes the condensation of tryptamine with secologanin into strictosidine. The gene encoding STR targeted to different subcellular compartments was transiently expressed in the tobacco leaves. In vitro STR enzymatic activity was measured by the depletion of tryptamine indicated by fluorescence. The results showed that the recombinant STR was effectively expressed as soluble protein in three subcellular compartments-chloroplast, vacuole and endoplasmic reticulum in the leaves of tobacco by Western blot analysis and STR enzymatic assay.
- Subjects :
- Blotting, Western
Carbon-Nitrogen Lyases metabolism
Chloroplasts enzymology
Endoplasmic Reticulum enzymology
Plant Leaves enzymology
Plants, Genetically Modified
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Nicotiana enzymology
Transfection methods
Transformation, Genetic
Tryptamines metabolism
Vacuoles enzymology
Carbon-Nitrogen Lyases genetics
Plant Leaves genetics
Nicotiana genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0582-9879
- Volume :
- 34
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 12417910