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[Expression of energy metabolism-related genes in myocardial tissues of rats with diabetic cardiomyopathy].
- Source :
-
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2002 Nov; Vol. 22 (11), pp. 1009-10. - Publication Year :
- 2002
-
Abstract
- Objective: To study the pathogenesis of diabetic cardiomyopathy using DNA microarray.<br />Methods: Experimental rats were grouped into a diabetic cardiomyopathy and a normal control group. The cDNA probes were prepared by labeling the mRNA extracted from normal tissue and tissue of diabetic cardiomyopathy with Cy3-dUTP and Cy5-dUTP respectively through reverse transcription. DNA microarray were constructed by spotting PCR products of 4 000 rat cDNAs onto a specially treated glass slides, and were then hybridized against the cDNA probes followed by fluorescent signals scanning.<br />Results: The expression of energy metabolism-related genes were lower in the tissues of diabetic cardiomyopathy than in normal tissue.<br />Conclusion: The energy metabolism disorder may play an important role in the pathogenesis of diabetic cardiomyopathy.
- Subjects :
- Animals
Cardiomyopathies genetics
Diabetes Complications
Diabetes Mellitus genetics
Disease Models, Animal
Gene Expression Profiling
Male
Oligonucleotide Array Sequence Analysis
Rats
Rats, Sprague-Dawley
Cardiomyopathies metabolism
Diabetes Mellitus metabolism
Energy Metabolism genetics
Gene Expression
Myocardium metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-2588
- Volume :
- 22
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
- Publication Type :
- Academic Journal
- Accession number :
- 12433633