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Differences in expression of toll-like receptors and their reactivities in dendritic cells in BALB/c and C57BL/6 mice.
- Source :
-
Infection and immunity [Infect Immun] 2002 Dec; Vol. 70 (12), pp. 6638-45. - Publication Year :
- 2002
-
Abstract
- We have previously reported that differences in early production of interleukin 12 (IL-12) by dendritic cells (DC) underlies the difference between the susceptibilities to Listeria monocytogenes of C57BL/6 and BALB/c mice. To elucidate mechanisms for the different abilities of DC to produce cytokine in C57BL/6 and BALB/c mice, we examined Toll-like receptor (TLR) expression by DC and their responses in vitro to known microbial ligands for TLRs. We found that DC isolated from the spleens of naive C57BL/6 mice preferentially expressed TLR9 mRNA, whereas DC from naive BALB/c mice strongly expressed TLR2, -4, -5, and -6 mRNAs. C57BL/6 DC produced a higher level of IL-12p40 in response to the ligands for TLR4 (lipopolysaccharide), TLR2 (lipoprotein), and TLR9 (CpG), whereas BALB/c DC responded to these ligands by producing a larger amount of monocyte chemoattractant protein 1. C57BL/6 DC expressed higher levels of CD40 and Stat4 than BALB/c DC did, suggesting that naive C57BL/6 mice contained more-mature subsets of DC than naive BALB/c mice. Differences in reactivities of DC to microbial molecules through TLRs may be associated with susceptibility and resistance to Listeria infection in BALB/c and C57BL/6 mice.
- Subjects :
- Animals
Dendritic Cells metabolism
Disease Susceptibility
Listeria monocytogenes metabolism
Listeriosis microbiology
Male
Membrane Glycoproteins genetics
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Receptors, Cell Surface genetics
Species Specificity
Spleen cytology
Spleen immunology
Toll-Like Receptor 2
Toll-Like Receptor 4
Toll-Like Receptors
Dendritic Cells immunology
Drosophila Proteins
Listeria monocytogenes pathogenicity
Listeriosis immunology
Membrane Glycoproteins metabolism
Receptors, Cell Surface metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 70
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 12438336
- Full Text :
- https://doi.org/10.1128/IAI.70.12.6638-6645.2002