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Expression of MMP-2,TIMP-2 protein and the ratio of MMP-2/TIMP-2 in gallbladder carcinoma and their significance.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2002 Dec; Vol. 8 (6), pp. 1138-43. - Publication Year :
- 2002
-
Abstract
- Aim: To study the correlation between expression of MMP-2, TIMP-2 protein and the ratio of MMP-2/TIMP-2 and clinical-pathological parameters of patients with gallbladder carcinoma.<br />Methods: Carcinomas (n=45) and polypoid lesions (n=15) of the gallbladder were studied for the expression of MMP-2 and TIMP-2 protein by immunohistochemical avidin-biotin-complex method and image analysis. Clinical-pathological data of patients with gallbladder carcinoma such as histological type, grade of differentiation, level of infiltration, liver invasion and lymph node involvement, etc, were recorded.<br />Results: There was significant difference between the average level (1.123+/-0.108 vs 1.030+/-0.054, P=0.002) of MMP-2, the ratio (1.050+/-0.013 vs 0.937+/-0.078, P=0.003) of MMP-2/TIMP-2 in gallbladder carcinomas and in polypoid lesions of the gallbladder. Significant difference was found between the expression of MMP-2 in early stage and advanced tumors, but there was no correlation between MMP-2 protein expression and histological type, differentiation degree, infiltration level, lymph node involvement or liver invasion. Although no difference was observed between TIMP-2 expression and histological type or differentiation degree, significant difference was found between TIMP-2 expression and different Nevin stage, infiltration level, local lymph node involvement or liver invasion (1.168+/-0.067 vs 1.048+/-0.075, 1.170+/-0.062 vs 1.039+/-0.069, 1.039+/-0.076 vs 1.147+/-0.083, 1.048+/-0.074 vs 1.103+/-0.095, P<0.05). MMP-2/TIMP-2 ratio did not correlate with histological type, grade of differentiation and liver invasion, but significant differences were found between MMP-2/TIMP-2 ratio and different Nevin stage, infiltration level and lymph node involvement in patients with carcinoma of gallbladder.<br />Conclusion: TIMP-2 and MMP-2/TIMP-2 ratio could reflect more accurately biological characteristic of gallbladder carcinoma and MMP-2/TIMP-2 ratio might be a new significant marker in early diagnosis, in the judgment of invasion or metastasis and the estimate of prognosis in patients with gallbladder carcinomas.
- Subjects :
- Adenocarcinoma metabolism
Adenocarcinoma pathology
Adenocarcinoma secondary
Adult
Aged
Aged, 80 and over
Carcinoma, Papillary metabolism
Carcinoma, Papillary pathology
Carcinoma, Papillary secondary
Case-Control Studies
Female
Gallbladder Neoplasms pathology
Humans
Lymphatic Metastasis
Male
Middle Aged
Neoplasm Invasiveness
Polyps metabolism
Polyps pathology
Gallbladder Neoplasms metabolism
Matrix Metalloproteinase 2 metabolism
Tissue Inhibitor of Metalloproteinase-2 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1007-9327
- Volume :
- 8
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 12439941
- Full Text :
- https://doi.org/10.3748/wjg.v8.i6.1138