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Citicoline increases glutathione redox ratio and reduces caspase-3 activation and cell death in staurosporine-treated SH-SY5Y human neuroblastoma cells.
- Source :
-
Brain research [Brain Res] 2002 Dec 06; Vol. 957 (1), pp. 84-90. - Publication Year :
- 2002
-
Abstract
- Citicoline, or CDP-choline, is an essential endogenous intermediate in the biosynthesis of phosphatidylcholine that may act as a neuroprotector in several models of neurodegeneration. The present study analyses the effects of citicoline in the paradigm of staurosporine-induced cell death in human SH-SY5Y neuroblastoma cells. Citicoline reduces apoptosis induced by 100 nM staurosporine for 12 h in SH-SY5Y cells. This effect is higher with pre-treatment of 60 mM citicoline for 24 h after staurosporine challenge. Moreover, citicoline treatment restores glutathione redox ratio diminished after staurosporine challenge. Finally, citicoline also reduces the expression levels of active caspase-3 and specific PARP-cleaved products of 89 kDa resulting from staurosporine exposure when citicoline is added to the culture medium 24 h before staurosporine. These findings demonstrate that citicoline affects the staurosporine-induced apoptosis cell-signalling pathway by interacting with the glutathione system and by inhibiting caspase-3 in SH-SY5Y human neuroblastoma cells.
- Subjects :
- Apoptosis drug effects
Blotting, Western
Caspase 3
Cell Death drug effects
Cell Line
Cytidine Diphosphate Choline metabolism
Humans
Neuroblastoma
Nootropic Agents metabolism
Oxidation-Reduction drug effects
Tumor Cells, Cultured
Caspase Inhibitors
Caspases metabolism
Cytidine Diphosphate Choline pharmacology
Enzyme Inhibitors pharmacology
Glutathione drug effects
Glutathione metabolism
Nootropic Agents pharmacology
Staurosporine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-8993
- Volume :
- 957
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 12443983
- Full Text :
- https://doi.org/10.1016/s0006-8993(02)03605-3