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Expression and somatic mutation on androgen receptor gene in prostate cancer.
- Source :
-
International journal of urology : official journal of the Japanese Urological Association [Int J Urol] 2002 Oct; Vol. 9 (10), pp. 545-53. - Publication Year :
- 2002
-
Abstract
- Background: Lack of androgen receptor (AR) expression or mutation on the AR gene creates the tendency for androgen independence and progression of prostate cancer. However, the association between the progression and AR expression or mutations is still controversial. In this study, we evaluated the prognostic significance of AR expression and mutations in prostate cancers.<br />Methods: Forty-two prostate adenocarcinomas and three lymph node metastatic lesions sampled prior to hormonal therapy were included in this study; AR expression was analyzed immunohistochemically using an antibody against AR and the result was scored as the percentage of AR-positive tumor cells in the total tumor cells. Polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) analysis and DNA sequencing were used to detect AR mutations.<br />Results: Our study revealed the average AR expression in the prostate adenocarcinoma was 52.2 +/- 27.1%, which was significantly lower than that in the adjacent non-tumorous prostate tissue (68.3 +/- 18.3% in average) (P < 0.001). A significant correlation was obtained between progression-free survival and AR expression (P < 0.01). By SSCP analysis, three silent mutations (T649T, E709E and E711E) were detected in three separate prostate carcinomas.<br />Conclusion: : We conclude that AR expression is a useful prognostic indicator for tumor progression. Androgen receptor mutation may be an uncommon molecular event in untreated prostate cancer in Japanese men.
- Subjects :
- Adenocarcinoma metabolism
Adenocarcinoma pathology
Aged
Aged, 80 and over
Disease Progression
Humans
Immunohistochemistry
Male
Middle Aged
Neoplasm Metastasis
Neoplasm Recurrence, Local metabolism
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Prognosis
Prostatic Neoplasms metabolism
Prostatic Neoplasms pathology
Receptors, Androgen metabolism
Sequence Analysis, DNA
Adenocarcinoma genetics
Gene Expression genetics
Mutation genetics
Neoplasm Recurrence, Local genetics
Prostatic Neoplasms genetics
Receptors, Androgen genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0919-8172
- Volume :
- 9
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of urology : official journal of the Japanese Urological Association
- Publication Type :
- Academic Journal
- Accession number :
- 12445232
- Full Text :
- https://doi.org/10.1046/j.1442-2042.2002.00514.x