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[Relationship between expressions of telomerase genes and apoptosis related genes in mammary ductal atypical hyperplasia].
- Source :
-
Ai zheng = Aizheng = Chinese journal of cancer [Ai Zheng] 2002 May; Vol. 21 (5), pp. 484-8. - Publication Year :
- 2002
-
Abstract
- Background & Objective: Recent studies showed that the activating of telomerase and excessive expression of apoptosis suppressor genes were related to the development of many tumors. This study was designed to investigate the expression of telomerase genes (hTR, hTRT) and apoptosis related genes (p53, bcl-2) in mammary atypical ductal hyperplasia for exploring the change of telomerase activity and apoptosis related genes in the process of mammary ductal dysplasia to malignant transformation.<br />Method: Expressions of telomerase genes (hTR, hTRT) and apoptosis related genes (p53, bcl-2) were detected by in situ hybridization and expression of the mutant p53 protein was detected by immunohistochemistry were detected in 44 patients with mammary atypical ductal hyperplasia, those expression were compared with those of the 6 benign hyperplasia and 26 breast carcinoma.<br />Result: High expressions of telomerase genes (hTR, hTRT mRNA) in severe atypical ductal hyperplasia (60.9%, 52.1%) is of significantly difference from that in mild-medium atypical ductal hyperplasia (22.2%, 11.1%; 33.3%, 25.0%) and breast cancer (88.5%, 80.8%). The upgrading of atypia link with decreased expression of wild p53 mRNA (mild: 55.6%; medium: 41.7%; severe: 26.1%) and increased expression of the mutant p53 protein (mild: 11.1%; medium: 25.0%; severe: 34.8%). As for bcl-2 mRNA, it shows moderate expression, especially in severe atypical ductal hyperplasia.<br />Conclusion: Our study revealed significant correlation between expressions of telomerase genes (hTR, hTRT) and the state of malignant transformation in mammary atypical ductal hyperplasia. Decreased expression of wild p53 gene, increased expression of mutant p53 protein, and overexpression of bcl-2 gene were associated with telomerase.
- Subjects :
- Breast Neoplasms metabolism
Breast Neoplasms pathology
Carcinoma, Ductal, Breast metabolism
Carcinoma, Ductal, Breast pathology
DNA-Binding Proteins
Female
Humans
Apoptosis
Breast Neoplasms genetics
Carcinoma, Ductal, Breast genetics
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Proto-Oncogene Proteins c-bcl-2 genetics
Telomerase genetics
Tumor Suppressor Protein p53 genetics
Subjects
Details
- Language :
- Chinese
- Volume :
- 21
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Ai zheng = Aizheng = Chinese journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 12452037