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Decrease of the D4 dopamine receptor messenger RNA expression in lymphocytes from patients with major depression.
- Source :
-
Progress in neuro-psychopharmacology & biological psychiatry [Prog Neuropsychopharmacol Biol Psychiatry] 2002 Oct; Vol. 26 (6), pp. 1155-60. - Publication Year :
- 2002
-
Abstract
- Objectives: The evaluation of the possible role of dopamine in psychiatric disorders has been limited by the relative inadequacy of tools. A tempting approach to examine alterations of dopaminergic system in major depression is to examine the expression of dopamine receptors in peripheral blood mononuclear cells (PBMC).<br />Methods: D4 dopamine receptor (D4DR) messenger RNA (mRNA) expression in PBMC from 12 patients with major depressive disorder was examined before and after an 8-week treatment with paroxetine at 20-50 mg/day. Ten healthy subjects were analyzed in parallel. The relative content of D4DR mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). using beta-actin as internal standard.<br />Results: D4DR mRNA levels were significantly decreased in untreated depressed patients as compared to controls. D4DR mRNA expression returned to control levels after paroxetine treatment, when patients achieved a significant improvement of depressive symptoms.<br />Conclusions: Results of our study suggest the role of PBMC D4DR mRNA expression as a peripheral marker of the central dopaminergic function in major depression.
- Subjects :
- Actins biosynthesis
Adolescent
Adult
Aged
Antidepressive Agents, Second-Generation therapeutic use
Biomarkers
Densitometry
Depressive Disorder, Major drug therapy
Depressive Disorder, Major psychology
Female
Humans
Lymphocytes drug effects
Male
Middle Aged
Monocytes drug effects
Monocytes metabolism
Oligonucleotide Probes
Paroxetine therapeutic use
Psychiatric Status Rating Scales
Receptors, Dopamine D4
Reverse Transcriptase Polymerase Chain Reaction
Selective Serotonin Reuptake Inhibitors therapeutic use
Depressive Disorder, Major blood
Lymphocytes metabolism
RNA, Messenger biosynthesis
Receptors, Dopamine D2 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0278-5846
- Volume :
- 26
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Progress in neuro-psychopharmacology & biological psychiatry
- Publication Type :
- Academic Journal
- Accession number :
- 12452539
- Full Text :
- https://doi.org/10.1016/s0278-5846(02)00253-1