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Protection of coumarins against linoleic acid hydroperoxide-induced cytotoxicity.
- Source :
-
Chemico-biological interactions [Chem Biol Interact] 2003 Jan 06; Vol. 142 (3), pp. 239-54. - Publication Year :
- 2003
-
Abstract
- Coumarins comprise a group of natural phenolic compounds found in a variety of plant sources. Protective effects of coumarins against cytotoxicity induced by linoleic acid hydroperoxide were examined in cultured human umbilical vein endothelial cells. When the cells were incubated in medium supplemented with linoleic acid hydroperoxide and coumarins, esculetin (6,7-dihydroxycoumarin) and 4-methylesculetin protected cells from injury by linoleic acid hydroperoxide. Fraxetin and caffeic acid showed weak, but significant, protection. Esculin as well as esculetin and 4-methylesculetin were effective for protecting cells against linoleic acid hydroperoxide-induced cytotoxicity in the case of pretreatment for 24 h, however fraxetin and caffeic acid showed no protection. Since esculetin was detected after 24 h treatment with esculin, a sugar moiety in the esculin molecule appears to be hydrolyzed during pretreatment. Coumarins such as umbelliferone containing only one hydroxyl group showed no protective effect in pretreatment or concurrent treatment. Esculetin and 4-methylesculetin provided synergistic protection against cytotoxicity induced by linoleic acid hydroperoxide with alpha-tocopherol. Furthermore, the radical-scavenging ability of coumarins was examined in electron spin resonance spectrometry. Esucletin, 4-methylesculetin, fraxetin, and caffeic acid showed the quenching effect on the 1,1-diphenyl-2-picrylhydrazyl radical. These results indicate that the presence of an ortho catechol moiety in the coumarin molecules plays an important role in the protective activities against linoleic acid hydroperoixde-induced cytotoxicity.<br /> (Copyright 2002 Elsevier Science Ireland Ltd.)
- Subjects :
- Cell Survival drug effects
Cells, Cultured
Cinnamates pharmacology
Coumarins chemistry
Drug Synergism
Electron Spin Resonance Spectroscopy
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Free Radical Scavengers chemistry
Free Radical Scavengers pharmacology
Humans
Umbelliferones pharmacology
Coumarins pharmacology
Linolenic Acids antagonists & inhibitors
Linolenic Acids toxicity
Lipid Peroxides antagonists & inhibitors
Lipid Peroxides toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0009-2797
- Volume :
- 142
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Chemico-biological interactions
- Publication Type :
- Academic Journal
- Accession number :
- 12453663
- Full Text :
- https://doi.org/10.1016/s0009-2797(02)00077-7