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Differential regulation of TGA transcription factors by post-transcriptional control.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2002 Dec; Vol. 32 (5), pp. 641-53. - Publication Year :
- 2002
-
Abstract
- Transcription factors often belong to multigene families and their individual contribution in a particular regulatory network remains difficult to assess. We show here that specific members from a family of conserved Arabidopsis bZIP transcription factors, the TGA proteins, are regulated in their protein stability by developmental stage-specific proteolysis. Using GFP fusions of three different Arabidopsis TGA factors that represent members of distinct subclasses of the TGA factor family, we demonstrate that two of these TGA proteins are specifically targeted for proteolysis in mature leaf cells. Using a supershift gel mobility assay, we found evidence for similar regulation of the cognate proteins as compared to the GFP fusion proteins expressed under the cauliflower mosaic virus (CaMV) 35S promoter. Using various inhibitors, we showed that the expression of at least one of these three TGA factors could be stabilized by inhibition of proteasome-mediated proteolysis. This study indicates that TGA transcription factors may be regulated by distinct pathways of targeted proteolysis that can serve to modulate the contribution of specific members of a multigene family in complex regulatory pathways.
- Subjects :
- Arabidopsis genetics
Arabidopsis growth & development
Arabidopsis metabolism
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Basic-Leucine Zipper Transcription Factors
DNA-Binding Proteins genetics
Gene Expression Regulation, Plant
Genes, Plant genetics
Peptide Hydrolases metabolism
Plant Proteins genetics
Plants, Genetically Modified
Nicotiana cytology
Nicotiana genetics
Nicotiana metabolism
Transcription Factors genetics
DNA-Binding Proteins metabolism
Plant Proteins metabolism
Protein Processing, Post-Translational
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0960-7412
- Volume :
- 32
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 12472682
- Full Text :
- https://doi.org/10.1046/j.1365-313x.2002.01461.x