Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase.

Authors :: Ruiz C
Blanco A
Pastor FI
Diaz P
Source :: FEMS microbiology letters [FEMS Microbiol Lett] 2002 Dec 17; Vol. 217 (2), pp. 263-7.
Publication Year :: 2002
Abstract: The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C(4)-C(8)) substrates, and poor activity on C(18) substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases.<br /> (Copyright 2002 Federation of European Microbiological Societies)

Subjects :: Base Sequence
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Hot Temperature
Hydrogen-Ion Concentration
Lipolysis
Bacillus megaterium enzymology
Bacillus megaterium metabolism
Bacterial Proteins genetics
Genes, Bacterial
Lipase genetics

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