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[Chemokine receptor expression of hepatitis B virus-specific CD8+ lymphocyte in chronic B viral infection].
- Source :
-
Taehan Kan Hakhoe chi = The Korean journal of hepatology [Taehan Kan Hakhoe Chi] 2002 Dec; Vol. 8 (4), pp. 363-70. - Publication Year :
- 2002
-
Abstract
- Background/aims: The protective role of HBV-specific CD8+ cells is dependent on their ability to efficiently migrate to the infected liver, where they may exert an effector function. The migratory behavior of CD8+ cells is influenced by their expression of different chemokine receptors. This study was intended to analyse the pattern of chemokine receptor expression of HBV specific CD 8+ cells in chronic B viral infection.<br />Methods: We analysed the CCR5 and CCR3 profile of HBV-specific CD8+ cells isolated from the blood and liver of patients with different patterns of HBV infection. Purified T cells were stained directly ex vivo, or after antigen-specific stimulation, using HBV peptide-specific HLA tetramers and monoclonal antibodies to CD8, CCR5 and CCR3, with analysis by flow cytometry.<br />Results: In patients with chronic hepatitis B characterised by low levels of virus (serum HBV DNA <0.5 pg/mL) and minimal liver inflammation, analysis of circulating and intrahepatic CD8+ cells demonstrated that liver infiltrating Tc18-27-specific cells were preferentially CCR5+ (up to 80% of HBV-specific CD8+ cells), in contrast to cells of the same specificity within the circulating compartment (up to 35% of HBV-specific CD8+ cells). Furthermore, CCR3 was expressed by about 10% of Tc18-27+ cells infiltrating the liver, but was absent from circulating cells. Following HBV-specific stimulation in vitro the CCR5 expression of circulating Tc18-27-specific cells was up-regulated, to levels found in liver infiltrating cells, whereas CCR3 expression was unchanged.<br />Conclusions: The chemokine receptor profile of HBV-specific CD8+ cells is influenced by the anatomical site of these cells, and the clinical pattern of disease. The ability of circulating HBV-specific CD8+ cells of patients with low replicating virus to upregulate CCR5 suggests that these cells may respond to increases in virus replication by efficiently migrating into the infected liver.
- Subjects :
- CD8-Positive T-Lymphocytes immunology
Hepatitis B virus immunology
Hepatitis B, Chronic pathology
Humans
Liver pathology
Receptors, CCR3
Receptors, CCR5 metabolism
T-Cell Antigen Receptor Specificity
CD8-Positive T-Lymphocytes metabolism
Hepatitis B, Chronic immunology
Receptors, Chemokine metabolism
Subjects
Details
- Language :
- Korean
- ISSN :
- 1226-0479
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Taehan Kan Hakhoe chi = The Korean journal of hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 12506240