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Human lactoferrin impairs virulence of Shigella flexneri.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2003 Jan 01; Vol. 187 (1), pp. 87-95. Date of Electronic Publication: 2002 Dec 13. - Publication Year :
- 2003
-
Abstract
- Lactoferrin is a glycoprotein present in most human mucosal secretions, including human milk. Lactoferrin is bacteriostatic in low iron media and, in some settings, bactericidal. Lactoferrin impairs ability of Shigella flexneri serotype 5 strain M90T to invade HeLa cells. To determine the mechanism by which lactoferrin decreases invasiveness of Shigella organisms, its effect on the major virulence proteins responsible for bacterial uptake by host cells was evaluated. Lactoferrin induced degradation of invasion plasmid antigens IpaB and, to a lesser extent, IpaC, the key proteins responsible for bacteria-directed phagocytosis by mammalian cells. The lipid A-binding N-terminal portion of lactoferrin (residues 1-33) induces release of invasion antigens but does not induce degradation of IpaBC. Lactoferrin does not directly degrade previously released invasion plasmid antigens but works by making IpaBC susceptible to breakdown by surface-expressed protease(s).
- Subjects :
- Antigens, Bacterial metabolism
Bacterial Adhesion drug effects
Bacterial Proteins metabolism
HeLa Cells
Humans
Lipid A metabolism
Plasmids
Protease Inhibitors pharmacology
Shigella flexneri growth & development
Shigella flexneri pathogenicity
Virulence
Lactoferrin pharmacology
Shigella flexneri drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 187
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 12508150
- Full Text :
- https://doi.org/10.1086/345875