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Very high-field EPR study of glycyl radical enzymes.
- Source :
-
Journal of the American Chemical Society [J Am Chem Soc] 2003 Jan 08; Vol. 125 (1), pp. 38-9. - Publication Year :
- 2003
-
Abstract
- This work shows that very high-field EPR spectroscopy allows a rather accurate determination of the g-tensor of protein radicals, including C-centered ones, and thus may be used as a probe for distinguishing a tyrosyl-, a glycyl-, or a tryptophanyl-radical. In this paper, we report the first complete analysis of the g-tensor of glycyl radical enzymes (anaerobic ribonucleotide reductase, pyruvate formate lyase, and benzylsuccinate synthase), thus providing new information on their EPR properties. Because the g-anisotropy is small, the complete resolution of the g-tensor could be only obtained at very high field (18.8 T).
Details
- Language :
- English
- ISSN :
- 0002-7863
- Volume :
- 125
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of the American Chemical Society
- Publication Type :
- Academic Journal
- Accession number :
- 12515500
- Full Text :
- https://doi.org/10.1021/ja026690j