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TRANCE together with IL-7 induces pre-B cells to proliferate.
- Source :
-
European journal of immunology [Eur J Immunol] 2003 Feb; Vol. 33 (2), pp. 334-41. - Publication Year :
- 2003
-
Abstract
- TRANCE (TNF-related activation-induced cytokine)-deficient mice completely lack osteoclasts, and develop severe osteopetrosis. These mice also show a defect in their pre-B cell differentiation. In the present study, the expression of TRANCE was examined in pre-B cell lines using flow cytometry and reverse transcription-PCR. Three pre-B cell lines, 18-81, B3P816-1, and 38B9, expressed TRANCE on their surface, and two pre-B cell lines, 7OZ/3 and NFS5, at the late pre-B cell stage, expressed it at low levels, although their mRNA expression was normal. Another pre-B cell line, 38-C-13, at the intermediate stage between pre-B and immature B cells, did not express TRANCE. The IL-7-dependent pre-B cell line PreBR, which expresses the pre-B cell receptor on the cell surface, also expressed TRANCE. When differentiation of PreBR cells was induced in vitro by removing IL-7 from cultures, TRANCE expression dropped; it was restored by the addition of IL-7, suggesting that TRANCE functions in cooperation with IL-7. To examine the function of TRANCE, we introduced the TRANCE gene into PreBR cells and established two transfectants that constitutively expressed TRANCE, even in the absence of IL-7. In these transfectants, after removal of IL-7, the number of cells that succeeded in kappa chain rearrangement was decreased to one third; and CD40 expression decreased to less than one tenth. Moreover, the percentage of cells in the S/G2/M phase was increased by 50% over the mock transfectant. These findings indicate that, before kappa chain rearrangement occurs, TRANCE together with IL-7 induces pre-B cells to proliferate and makes this rearrangement more efficient.
- Subjects :
- Animals
B-Lymphocytes cytology
CD40 Antigens biosynthesis
CD40 Antigens genetics
Carrier Proteins genetics
Cell Cycle drug effects
Cell Division drug effects
Cell Line drug effects
Gene Expression Regulation drug effects
Gene Rearrangement, B-Lymphocyte, Light Chain drug effects
Glycoproteins metabolism
Hematopoietic Stem Cells cytology
Immunoglobulin kappa-Chains genetics
Membrane Glycoproteins genetics
Mice
Mice, Inbred BALB C
Osteoprotegerin
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
Receptors, Cytoplasmic and Nuclear metabolism
Receptors, Tumor Necrosis Factor
Recombinant Fusion Proteins metabolism
Recombinant Fusion Proteins physiology
Signal Transduction
Transfection
B-Lymphocytes drug effects
Carrier Proteins physiology
Hematopoietic Stem Cells drug effects
Interleukin-7 pharmacology
Membrane Glycoproteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 33
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12548564
- Full Text :
- https://doi.org/10.1002/immu.200310007