Back to Search
Start Over
Delivery of a growth factor fusion protein having collagen-binding activity to wound tissues.
- Source :
-
Artificial organs [Artif Organs] 2003 Feb; Vol. 27 (2), pp. 147-54. - Publication Year :
- 2003
-
Abstract
- Recently, we established a collagen-binding growth factor consisting of epidermal growth factor and the fibronectin collagen-binding domain (FNCBD-EGF). FNCBD-EGF is a biologically active fusion protein that could stably bind to collagen materials, and exert its growth factor activity even after collagen binding. In this study, we investigated the concept that FNCBD moiety with high collagen affinity may enhance the effective local concentration of EGF at the site of administration in the following tissues: skin wounds, catheter-injured arteries, and hind limb muscles. In an animal model of impaired wound healing, application of FNCBD-EGF in combination with collagen gel induced granulation tissue formation in the wounds due to its sustained retention. In the injured artery, infused FNCBD-EGF remained bound to collagen exposed on the injured tissues even after blood circulation was restored. Injection of the fusion protein into the hind limbs revealed that our delivery system was effective for direct administration to muscular tissue.
- Subjects :
- Administration, Topical
Animals
Carotid Artery Injuries therapy
Diabetes Mellitus, Experimental physiopathology
Epidermal Growth Factor metabolism
Epidermal Growth Factor pharmacokinetics
Fibronectins metabolism
Fibronectins pharmacokinetics
Gels
Hindlimb
Immunohistochemistry
Injections, Intramuscular
Male
Mice
Mice, Inbred C57BL
Proliferating Cell Nuclear Antigen analysis
Protein Binding
Rabbits
Rats
Rats, Sprague-Dawley
Recombinant Fusion Proteins pharmacokinetics
Collagen metabolism
Drug Delivery Systems
Epidermal Growth Factor administration & dosage
Fibronectins administration & dosage
Recombinant Fusion Proteins administration & dosage
Wound Healing drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0160-564X
- Volume :
- 27
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Artificial organs
- Publication Type :
- Academic Journal
- Accession number :
- 12580771
- Full Text :
- https://doi.org/10.1046/j.1525-1594.2003.07009.x