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A new inhibitor of the transcription-termination factor Rho.

Authors :
Carrano L
Alifano P
Corti E
Bucci C
Donadio S
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2003 Mar 07; Vol. 302 (2), pp. 219-25.
Publication Year :
2003

Abstract

In this study we describe BI-K0058, a new inhibitor of the transcription-termination factor Rho belonging to a different chemical class from bicyclomycin, the only known antibiotic acting on Rho. BI-K0058 inhibits the poly(C)-dependent ATPase activity of Rho with an IC(50) of 25 microM as well as in vitro transcription-termination of two natural substrates, the Salmonella enterica hisG cistron and the f1 phage intergenic region. BI-K0058 does not affect photolabeling of Rho by ATP. The results of gel mobility shift experiments with a natural RNA substrate demonstrate that BI-K0058 inhibits the formation of the ATP-independent high affinity Rho-RNA complex.

Details

Language :
English
ISSN :
0006-291X
Volume :
302
Issue :
2
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
12604334
Full Text :
https://doi.org/10.1016/s0006-291x(03)00131-1