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A new inhibitor of the transcription-termination factor Rho.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2003 Mar 07; Vol. 302 (2), pp. 219-25. - Publication Year :
- 2003
-
Abstract
- In this study we describe BI-K0058, a new inhibitor of the transcription-termination factor Rho belonging to a different chemical class from bicyclomycin, the only known antibiotic acting on Rho. BI-K0058 inhibits the poly(C)-dependent ATPase activity of Rho with an IC(50) of 25 microM as well as in vitro transcription-termination of two natural substrates, the Salmonella enterica hisG cistron and the f1 phage intergenic region. BI-K0058 does not affect photolabeling of Rho by ATP. The results of gel mobility shift experiments with a natural RNA substrate demonstrate that BI-K0058 inhibits the formation of the ATP-independent high affinity Rho-RNA complex.
- Subjects :
- Adenosine Triphosphate metabolism
Anti-Bacterial Agents chemistry
Benzimidazoles chemistry
Bridged Bicyclo Compounds, Heterocyclic chemistry
Microbial Sensitivity Tests
Moraxella drug effects
Neisseria gonorrhoeae drug effects
RNA drug effects
RNA metabolism
Anti-Bacterial Agents pharmacology
Benzimidazoles pharmacology
Bridged Bicyclo Compounds, Heterocyclic pharmacology
Rho Factor antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 302
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 12604334
- Full Text :
- https://doi.org/10.1016/s0006-291x(03)00131-1