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Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China.
- Source :
-
Chinese medical journal [Chin Med J (Engl)] 2002 Dec; Vol. 115 (12), pp. 1881-2. - Publication Year :
- 2002
-
Abstract
- Objective: To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region.<br />Methods: The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed.<br />Results: Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands.<br />Conclusion: Nan Peng Lie Islands is tsutsugamushi disease nature foci.
Details
- Language :
- English
- ISSN :
- 0366-6999
- Volume :
- 115
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Chinese medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 12622944