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Withdrawal of TNF-alpha after the fifth day of differentiation of CD34+ cord blood progenitors generates a homogeneous population of Langerhans cells and delays their maturation.
- Source :
-
Experimental dermatology [Exp Dermatol] 2003 Feb; Vol. 12 (1), pp. 96-105. - Publication Year :
- 2003
-
Abstract
- Human cord blood CD34+ progenitors cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) generate a heterogeneous population of dendritic cells (DC), including Langerhans cells (LC). This combination of cytokines has been shown to be crucial for differentiation into LC. After day 5 of culture, TNF-alpha has been maintained in the medium in most studies despite the observation of spontaneous maturation of LC after day 12. Five-day samples of in vitro differentiated LC were cultured in parallel with or without TNF-alpha. The absence of TNF-alpha was shown to: (1) slow down proliferation without triggering apoptotic cell death, (2) enhance the percentage of LC, (3) delay or abrogate the expression of CD83, CD86, HLA-DR and CD208 molecules, and (4) maintain endocytosis by receptor and macropinocytosis. The withdrawal of TNF-alpha abrogated the spontaneous synthesis of matrix metalloproteinases. At day 12, TNF-alpha-deprived LC were less efficient in allogeneic T cell activation than LC cultivated with TNF-alpha. These data indicate that the suppression of TNF-alpha after day 5 maintains cells in an immature state and provides a population with 80% of LC at day 12.
- Subjects :
- Cell Differentiation drug effects
Cell Division drug effects
Cell Movement drug effects
Cellular Senescence
Drug Administration Schedule
Gelatinases metabolism
Humans
Langerhans Cells cytology
Langerhans Cells enzymology
Langerhans Cells physiology
Lymphocyte Culture Test, Mixed
Time Factors
Antigens, CD34 blood
Hematopoietic Stem Cells cytology
Hematopoietic Stem Cells physiology
Tumor Necrosis Factor-alpha administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0906-6705
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Experimental dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 12631252
- Full Text :
- https://doi.org/10.1034/j.1600-0625.2003.00043.x