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Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) forms complexes with a cellular anti-apoptosis protein Bcl-2 or its EBV counterpart BHRF1 through HS1-associated protein X-1.
- Source :
-
Microbiology and immunology [Microbiol Immunol] 2003; Vol. 47 (1), pp. 91-9. - Publication Year :
- 2003
-
Abstract
- Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP) plays a critical role in EBV-induced transformation. An earlier report (Y. Kawaguchi et al., J. Virol. 74: 10104-10111, 2000) showed that EBNA-LP interacts with a cellular protein HS1-associated protein X-1 (HAX-1). The predicted amino acid sequence of HAX-1 exhibits similarity to that of another cellular protein Nip3 which has been shown to interact with cellular and viral anti-apoptotic proteins such as Bcl-2 and BHRF1, an EBV homolog of Bcl-2. Here we investigated whether HAX-1, like Nip3, interacts with Bcl-2 proteins and report the following. (i) A purified chimeric protein consisting of gluthathione S-transferase (GST) fused to BHRF1 (GST-BHRF1) or Bcl-2 (GST-Bcl-2) specifically pulled down HAX-1 transiently expressed in COS-7 cells. (ii) GST-BHRF1 or GST-Bcl-2 was not able to pull down EBNA-LP transiently expressed in COS-7 cells, whereas each of the GST fusion proteins formed complexes with EBNA-LP in the presence of RAX-1. These results indicated that EBNA-LP interacts with the viral and cellular Bcl-2 proteins through HAX-1, suggesting that EBNA-LP possesses a potential function in the regulation of apoptosis in EBV-infected cells.
- Subjects :
- Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Blotting, Western
COS Cells
Chlorocebus aethiops
Epstein-Barr Virus Nuclear Antigens immunology
Escherichia coli genetics
Herpesvirus 4, Human immunology
Molecular Sequence Data
Proteins immunology
Proto-Oncogene Proteins c-bcl-2 immunology
Recombinant Proteins genetics
Sequence Alignment
Transfection
Viral Proteins immunology
Apoptosis physiology
Epstein-Barr Virus Nuclear Antigens metabolism
Herpesvirus 4, Human metabolism
Proteins metabolism
Proto-Oncogene Proteins c-bcl-2 metabolism
Viral Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0385-5600
- Volume :
- 47
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbiology and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12636258
- Full Text :
- https://doi.org/10.1111/j.1348-0421.2003.tb02790.x