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Establishment of an enzyme-linked immunosorbent assay for detection of hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in Escherichia coli.
- Source :
-
Experimental animals [Exp Anim] 2003 Jan; Vol. 52 (1), pp. 25-30. - Publication Year :
- 2003
-
Abstract
- A recombinant nucleocapsid protein of Hantaan virus (HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay (IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR-11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples.
- Subjects :
- Animals
Biomarkers blood
Female
Fluorescent Antibody Technique, Indirect
Rats
Rats, Wistar
Sensitivity and Specificity
Antibodies, Viral blood
Bacterial Proteins
Enzyme-Linked Immunosorbent Assay methods
Escherichia coli
Orthohantavirus immunology
Hantavirus Infections diagnosis
Nucleocapsid Proteins
Recombinant Proteins
Serologic Tests methods
Subjects
Details
- Language :
- English
- ISSN :
- 1341-1357
- Volume :
- 52
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Experimental animals
- Publication Type :
- Academic Journal
- Accession number :
- 12638233
- Full Text :
- https://doi.org/10.1538/expanim.52.25