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Xite, X-inactivation intergenic transcription elements that regulate the probability of choice.
- Source :
-
Molecular cell [Mol Cell] 2003 Mar; Vol. 11 (3), pp. 731-43. - Publication Year :
- 2003
-
Abstract
- Allelic expression differences contribute to phenotypic variation. In X chromosome inactivation (XCI), unfavorable XCI ratios promote X-linked disease penetrance in females. During XCI, one X is randomly silenced by Xist. X chromosome choice is determined by asymmetric expression of Tsix whose antisense action represses Xist. Here, we discover a cis element in the mouse X-inactivation center that regulates Tsix. Xite harbors intergenic transcription start sites and DNaseI hypersensitive sites with allelic differences. At the onset of XCI, deleting Xite downregulates Tsix in cis and skews XCI ratios, suggesting that Xite promotes Tsix persistence on the active X. Truncating Xite RNA is inconsequential, indicating that Xite action does not require intact transcripts. We propose that allele-specific Xite action promotes Tsix asymmetry and generates X chromosome inequality. Therefore, Xite is a candidate for the Xce, the classical modifier of XCI ratios.
- Subjects :
- Alleles
Animals
Bacterial Outer Membrane Proteins genetics
Base Sequence
CpG Islands
Deoxyribonuclease I metabolism
Down-Regulation
Exons
Female
Gene Deletion
Genes, Regulator
In Situ Hybridization, Fluorescence
Male
Mice
Mice, Knockout
Models, Genetic
Molecular Sequence Data
Protein Binding
RNA metabolism
RNA, Long Noncoding
RNA, Messenger metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic
Escherichia coli Proteins
Gene Expression Regulation
RNA, Untranslated genetics
Receptors, Virus
Transcription Factors genetics
X Chromosome
Subjects
Details
- Language :
- English
- ISSN :
- 1097-2765
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular cell
- Publication Type :
- Academic Journal
- Accession number :
- 12667455
- Full Text :
- https://doi.org/10.1016/s1097-2765(03)00063-7