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Nimesulide inhibits proliferation via induction of apoptosis and cell cycle arrest in human gastric adenocarcinoma cell line.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2003 May; Vol. 9 (5), pp. 915-20. - Publication Year :
- 2003
-
Abstract
- Aim: To evaluate the potential role of Nimesulide, a selective COX-2 inhibitor, in proliferation and apoptosis of gastric adenocarcinoma cells SGC7901.<br />Methods: Cell counts and MTT assay were used to quantify the influence of Nimesulide in the proliferation of SGC7901 cells. Transmission electron microscopy and flow cytometry were used to observe the induction of Nimesulide the apoptosis of SGC7901 cells and influence in the distribution of cell cycle. The expression of P27(kip1) protein was observed by immunocytochemical staining.<br />Results: SGC-7901 Cells treated with Nimesulide at various concentrations exhibited a profound dose- and time-dependent reduction in the proliferation rate over the 72 h test period. The highest survival rate of the cells was 78.7 %, but the lowest being 22.7 %. Nimesulide induced apoptosis of the cells in a dose-dependent and non-linear manner and increased the proportion of cells in the G(0)/G(1) phase and decreased the proportion in the S and G(2)/M phase of the cell cycle. Meanwhile, Nimesulide could up-regulate the expression of P27(kip1) protein.<br />Conclusion: The induction of apoptosis and cell cycle arrest are both anti-proliferative responses that likely contribute to the antineoplastic action of nimesulide on SGC-7901 cells. The up-regulation of P27(kip1) gene may contribute to the accumulation of these cells in the G(0)/G(1) phase following treatment with Nimesulide. Selective COX-2 inhibitor may be a new channel of the chemoprevention and chemotherapy for gastric carcinoma.
- Subjects :
- Adenocarcinoma metabolism
Apoptosis drug effects
Cell Cycle drug effects
Cell Cycle Proteins metabolism
Cyclin-Dependent Kinase Inhibitor p27
Cyclooxygenase Inhibitors pharmacology
Humans
Immunohistochemistry
Microscopy, Electron
Stomach Neoplasms metabolism
Tumor Cells, Cultured
Tumor Suppressor Proteins metabolism
Adenocarcinoma drug therapy
Adenocarcinoma pathology
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Cell Division drug effects
Stomach Neoplasms drug therapy
Stomach Neoplasms pathology
Sulfonamides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1007-9327
- Volume :
- 9
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 12717830
- Full Text :
- https://doi.org/10.3748/wjg.v9.i5.915