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Matrix metalloproteinases-2 and -9 are secreted from human fibroblasts.
- Source :
-
Acta dermato-venereologica [Acta Derm Venereol] 2003; Vol. 83 (2), pp. 105-7. - Publication Year :
- 2003
-
Abstract
- To investigate the pathophysiological role of fibroblasts in tissue metabolism, we analysed gelatin-degrading enzymes secreted from skin cells in culture. Using a gelatin-zymography method, matrix metalloproteinase (MMP)-2 was constitutively secreted from fibroblasts, whereas both MMP-2 and MMP-9 were secreted from keratinocytes. MMP-9 expression by fibroblasts was induced by the addition of transforming growth factor (TGF)-beta1 or tumour necrosis factor (TNF)-alpha to the cultures. Moreover, TGF-beta1, TNF-alpha, or epidermal growth factor augmented the secretion of MMP-2 from fibroblasts. These results indicate that MMP-9 together with MMP-2 secreted from fibroblasts could play important roles in tissue metabolism, including cytokine-induced inflammation.
- Subjects :
- Cell Culture Techniques
Epidermal Growth Factor pharmacology
Fibroblasts drug effects
Humans
Keratinocytes drug effects
Keratinocytes metabolism
Skin drug effects
Skin metabolism
Transforming Growth Factor beta pharmacology
Transforming Growth Factor beta1
Tumor Necrosis Factor-alpha pharmacology
Fibroblasts metabolism
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0001-5555
- Volume :
- 83
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Acta dermato-venereologica
- Publication Type :
- Academic Journal
- Accession number :
- 12735637
- Full Text :
- https://doi.org/10.1080/00015550310007436