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EBV specific antibody-based and DNA-based assays in serologic diagnosis of nasopharyngeal carcinoma.
- Source :
-
International journal of cancer [Int J Cancer] 2003 Jul 10; Vol. 105 (5), pp. 706-9. - Publication Year :
- 2003
-
Abstract
- We assessed 5 EBV specific assays for their capacity to effect serologic diagnosis of suspected NPC. The assays were the immunofluorescent assays, VCA IgA and EA IgA, the enzyme-linked immunosorbent assays specific for EBNA 1 IgA or zta IgG and an EBV DNA assay. Serum samples were taken from 218 symptomatic NPC patients presenting consecutively at a public hospital in Hong Kong, 51 of whom were subsequently diagnosed as having NPC; 4 had EBV-associated lung cancer with similar serology as NPC. The remaining patients included 23 who had other cancers and 140 who had other diseases. Objectives of serodiagnosis under such clinical settings, therefore, are to both exclude and predict a diagnosis of NPC. None of the assays individually can meet both requirements adequately, however. The difficulty was best overcome by combining EBNA 1 IgA and zta IgG. It was shown that 68.3% of the patients gave a confirmed test results, negative or positive, by both tests. A confirmed negative result was associated with a negative predictive value of 99.1%, providing a clear indication to exclude a diagnosis of NPC; a confirmed positive result was associated with a positive predictive value of 86.8%, providing a clear indication to proceed with diagnostic work-up of NPC. The remaining patients gave equivocal test results, being positive for one or the other test, which were associated with a positive predictive value of 43.3% and 24.2%, respectively.<br /> (Copyright 2003 Wiley-Liss, Inc.)
- Subjects :
- Antibody Specificity
Antigens, Viral immunology
Capsid Proteins immunology
Carcinoma blood
Carcinoma immunology
Carcinoma virology
Carcinoma, Squamous Cell blood
Carcinoma, Squamous Cell diagnosis
Carcinoma, Squamous Cell immunology
Carcinoma, Squamous Cell virology
DNA-Binding Proteins immunology
Epstein-Barr Virus Infections blood
Epstein-Barr Virus Infections immunology
Epstein-Barr Virus Nuclear Antigens immunology
Herpesvirus 4, Human genetics
Herpesvirus 4, Human immunology
Hong Kong
Humans
Immunoglobulin A blood
Immunoglobulin G blood
Lung Neoplasms blood
Lung Neoplasms diagnosis
Lung Neoplasms immunology
Lung Neoplasms virology
Nasopharyngeal Neoplasms blood
Nasopharyngeal Neoplasms immunology
Nasopharyngeal Neoplasms virology
Neoplasms blood
Neoplasms diagnosis
Neoplasms immunology
Neoplasms virology
Predictive Value of Tests
Trans-Activators immunology
Tumor Virus Infections blood
Tumor Virus Infections immunology
Viral Proteins immunology
Antibodies, Viral blood
Carcinoma diagnosis
DNA, Viral blood
Enzyme-Linked Immunosorbent Assay
Epstein-Barr Virus Infections diagnosis
Fluorescent Antibody Technique, Indirect
Herpesvirus 4, Human isolation & purification
Nasopharyngeal Neoplasms diagnosis
Tumor Virus Infections diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 105
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 12740922
- Full Text :
- https://doi.org/10.1002/ijc.11130