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[Types and emergence time of YMDD motif mutation in hepatitis B virus polymerase gene during lamivudine treatment].
- Source :
-
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology [Zhonghua Gan Zang Bing Za Zhi] 2003 May; Vol. 11 (5), pp. 302-4. - Publication Year :
- 2003
-
Abstract
- Objective: To study the types and emergence time of YMDD motif mutation in hepatitis B virus (HBV) polymerase gene during lamivudine treatment.<br />Methods: The serum samples were collected from 33 patients with HBV DNA rebounding and 2 non-responders after at least one year lamivudine treatment. HBV polymerase gene was amplificated by PCR, then the products were detected by restriction fragment length polymorphism (RFLP) and by direct sequence analysis.<br />Results: The variants with YMDD mutation were 14 out of the 35 patients. Mutation patterns detected in these patients included four YIDD, six YVDD, three YI/VDD and one YI/MDD. The mean emergence time of YMDD variants was 11.07+/-3.65 months after the treatment, and the earliest one and the latest one occurred 5 months and 17 months after the treatment respectively. The emergence times of YIDD, YVDD, YI/VDD were (10.00 +/- 1.41) months, (11.67 +/- 4.41) months and (13.33 +/- 3.31) months respectively, which had no statistical significance (F = 0.543, P < 0.05). Three patients treated with lamivudine 200 mg every day after the mutation were followed up for 6 months, whose HBV variants had not vanished.<br />Conclusions: There are many kinds of HBV variants after lamivudine treatment, including YIDD, YVDD, YI/VDD and YI/MDD. The emergence time of variants is quite variable between different types and the mean time is (11.07 +/- 3.65) months after treatment, and there is no relationship between the type of YMDD mutation and the time of lamivudine administration.
- Subjects :
- Adolescent
Adult
Aged
Amino Acid Motifs genetics
Child
Cloning, Molecular
DNA, Viral genetics
Drug Resistance, Viral genetics
Female
Hepatitis B virus drug effects
Hepatitis B virus enzymology
Hepatitis B, Chronic virology
Humans
Lamivudine pharmacology
Male
Middle Aged
RNA-Directed DNA Polymerase genetics
Time Factors
Gene Products, pol genetics
Hepatitis B virus genetics
Hepatitis B, Chronic drug therapy
Lamivudine therapeutic use
Mutation
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-3418
- Volume :
- 11
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 12773248