Back to Search
Start Over
Purification and characterization of Ulva pertusa Kjellm alkaline phosphatase.
- Source :
-
Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2003 May; Vol. 33 (2), pp. 113-23. - Publication Year :
- 2003
-
Abstract
- The activity of alkaline phosphatase (ALP, EC 3.1.3.1.) was found in seaweeds, including five kinds of green alga, eighteen kinds of red alga, and six kinds of brown alga, collected from the seaside of Dalian in China. The enzyme was purified 1230-fold from Ulva pertusa Kjellm. It had a specific activity of 48.6 U/mg protein and was proven to be homogeneous by SDS-PAGE with a subunit molecular mass of 19.5 kDa. The activity of ALP peaked at pH9.8, and was completely inhibited by DTT and partly by NBS. The Michaelis-Menten constant Km and the maximum reaction velocity Vmax, at pH 9.8 and 37 degrees C were 0.950 mM and 5.00 microM/min, respectively.
- Subjects :
- Alkaline Phosphatase drug effects
Chlorophyta enzymology
Chromatography, Affinity methods
Cobalt pharmacology
Copper pharmacology
Dithiothreitol pharmacology
Dose-Response Relationship, Drug
Edetic Acid pharmacology
Electrophoresis, Polyacrylamide Gel
Hydrogen-Ion Concentration
Kinetics
Magnesium pharmacology
Nickel pharmacology
Phaeophyceae enzymology
Rhodophyta enzymology
Seaweed enzymology
Spectrometry, Fluorescence
Temperature
Time Factors
Alkaline Phosphatase isolation & purification
Alkaline Phosphatase metabolism
Ulva enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1082-6068
- Volume :
- 33
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Preparative biochemistry & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 12784882
- Full Text :
- https://doi.org/10.1081/PB-120021436