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Regulation of the chromaffin granule catecholamine transporter in cultured bovine adrenal medullary cells: stimulus-biosynthesis coupling.
- Source :
-
Journal of neurochemistry [J Neurochem] 1992 Dec; Vol. 59 (6), pp. 2105-12. - Publication Year :
- 1992
-
Abstract
- The transsynaptic induction of the monoamine transporter present on the membrane of chromaffin granules was studied in primary cultures of dissociated bovine adrenomedullary cells submitted to a chronic secretory stimulation. The amount of the vesicular monoamine transporter was assayed by binding of the specific ligand [3H]-dihydrotetrabenazine. After several days of incubation in the presence of high potassium, the concentration of [3H]-dihydrotetrabenazine binding sites was increased by a 1.5-2.5 factor. This increase was smaller in the presence of the cholinergic agonist carbachol. The long-term inductions of the vesicular monoamine transporter, of tyrosine hydroxylase, and of acetylcholinesterase were of similar magnitude. Under the same conditions, we found no variation in either the activities of other catecholamine biosynthetic enzymes (dopamine beta-hydroxylase and DOPA decarboxylase), or in metabolic enzymes such as lactate dehydrogenase and cytochrome c oxidase, and a decrease in the cellular content of chromogranin A and cytochrome b-561. The induction of the vesicular monoamine transporter was inhibited by the calcium channel antagonists, fluspirilene and nifedipine, and was increased by the agonist Bay K 8644. It was abolished by cycloheximide and actinomycin D. These results indicate that calcium entry into chromaffin cells increases the synthesis of the vesicular monoamine transporter, presumably by transcriptional activation. Elevation of intracellular cyclic AMP concentration or activation of protein kinase C also induced an increase in the expression of the vesicular monoamine transporter. Our results confirm that components of storage vesicle membranes are differentially regulated in response to secretory stimulation, as are several cytosolic or intravesicular soluble proteins.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester pharmacology
Acetylcholinesterase analysis
Acetylcholinesterase metabolism
Adrenal Medulla chemistry
Animals
Biological Transport physiology
Carbachol pharmacology
Carrier Proteins analysis
Catecholamine Plasma Membrane Transport Proteins
Catecholamines pharmacokinetics
Cattle
Cells, Cultured
Chromaffin Granules metabolism
Chromaffin Granules ultrastructure
Chromogranin A
Chromogranins analysis
Chromogranins metabolism
Colforsin pharmacology
Cyclic AMP metabolism
Cycloheximide pharmacology
Dactinomycin pharmacology
Dopamine beta-Hydroxylase analysis
Dopamine beta-Hydroxylase metabolism
Membrane Proteins analysis
Membrane Proteins metabolism
Nifedipine pharmacology
Potassium pharmacology
Protein Kinase C metabolism
Tetrabenazine analogs & derivatives
Tetrabenazine metabolism
Time Factors
Tritium
Tyrosine 3-Monooxygenase analysis
Tyrosine 3-Monooxygenase metabolism
Adrenal Medulla cytology
Adrenal Medulla metabolism
Carrier Proteins metabolism
Chromaffin Granules chemistry
Membrane Transport Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 59
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 1279122
- Full Text :
- https://doi.org/10.1111/j.1471-4159.1992.tb10101.x